![]() ![]() For decades, disarmed T-DNAs, lacking the native tumorigenic genes, have been widely used for both transient and stable plant transformation ( Gelvin, 2005). Our findings enable efficient use of Agrobacterium-mediated transient transformation in Arabidopsis thaliana.Īgrobacterium tumefaciens possesses the unique ability to transfer its own DNA (transferred DNA, T-DNA) which integrates into plant genomes and alters the host plant environment to achieve ideal bacterial growth conditions ( Escobar and Dandekar, 2003 Tzfira et al., 2004). This transient expression system was successfully applied to analysis of the subcellular localization of a cyan fluorescent protein (CFP) fusion and a protein–protein interaction in Arabidopsis. When the transgenic plants were pretreated with DEX prior to infection with Agrobacterium carrying a β-glucuronidase (GUS, uidA) gene with an artificial intron and driven by the CaMV 35S promoter, transient GUS expression was dramatically enhanced compared to that in mock-pretreated plants. ![]() We used transgenic Arabidopsis plants that conditionally express AvrPto under the control of a dexamethasone (DEX)-inducible promoter. AvrPto is an effector protein from the bacterial plant pathogen Pseudomonas syringae that suppresses plant immunity by interfering with plant immune receptors. Here, we report a simple and robust method for Agrobacterium-mediated transient transformation in Arabidopsis. Previous studies suggested that this difficulty was caused by plant immune responses triggered by perception of Agrobacterium. However, in Arabidopsis this procedure has been challenging. ![]() Agrobacterium-mediated transient transformation of Nicotiana benthamiana by leaf infiltration has been widely used due to its ease and high efficiency. Agrobacterium tumefaciens-mediated transient transformation has been a useful procedure for characterization of proteins and their functions in plants, including analysis of protein–protein interactions. ![]()
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